Construction And Demonstration Of A Tandem Mass Spectrometer Based Instrument For Cold Ion Spectroscopy
نویسندگان
چکیده
Redwine, James Gerhardt. Ph.D., Purdue University, December 2013. Construction and Demonstration of a Tandem Mass Spectrometer Based Instrument for Cold Ion Spectroscopy. Major Professors: Scott McLuckey & Timothy Zwier. A new instrument incorporating ion trap based tandem mass spectrometry and cold ion UV and UV-IR double resonance spectroscopy has been developed at Purdue University for the study of gas phase, biologically relevant ions. The instrument incorporates multiple quadrupole linear ion trap mass analyzers to prepare and isolate the precursor ions as well as to analyze the resultant photofragments. A 22-pole ion trap cooled to 5 K via a closed cycle helium cryostat is used to cool the ions for spectroscopic interrogation. Results show the vibrational and rotational temperature of a wide assortment of ions to be 12±2 K. The capabilities of the instrument are confirmed using the protonated amino acid tyrosine (Tyr), following precedent established in the literature. The UV spectrum shows 12±2 K ion temperatures and signal to noise comparable to previous studies presented in the literature. A novel mode of UV photofragment spectroscopy is presented which allows for the observation of all photofragments, whereas previous methods observe the fragmentation of a single mass channel.
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